Jinghang Xie


Primary Appointment

Assistant Professor, Molecular Physiology and Biological Physics

Research Disciplines

Molecular Physiology and Biological Physics

Research Interests

Molecular Imaging; Cancer Immunology; Vaccines; Antibiotic Resistance.

Research Description

We forge collaborative efforts at the dynamic intersection of biology, chemistry, and medicine, propelling forward the boundaries of scientific exploration. Our work revolves around the development of cutting-edge imaging tools, empowering us to unravel the intricate mechanisms underlying immunological responses to vaccines and therapies, as well as the resilient resistance displayed by pathogenic bacteria towards antibiotics. Through our innovative approaches, we enable visualization and delve deep into the core of these phenomena, shedding light on their workings and opening new avenues for discovery and breakthroughs.

Personal Statement

We work on the interface of biology, chemistry, and medicine to develop imaging tools to visualize and investigate mechanisms of cancer response to immunotherapies.

Selected Publications


Dai, T., Xie, J., Buonomo, J. A., Moreno, A., Banaei, N., Bertozzi, C. R., & Rao, J. (2023). Bioluminogenic Probe for Rapid, Ultrasensitive Detection of ?-Lactam-Resistant Bacteria. ANALYTICAL CHEMISTRY, 95(18), 7329-7335. doi:10.1021/acs.analchem.3c00478

Yin, Q., Luo, W., Mallajosyula, V., Bo, Y., Guo, J., Xie, J., . . . Davis, M. M. M. (2023). A TLR7-nanoparticle adjuvant promotes a broad immune response against heterologous strains of influenza and SARS-CoV-2. NATURE MATERIALS, 22(3), 380-+. doi:10.1038/s41563-022-01464-2


Chen, M., Zhou, K., Dai, S. -Y., Tadepalli, S., Balakrishnan, P. B., Xie, J., . . . Rao, J. (2022). In vivo bioluminescence imaging of granzyme B activity in tumor response to cancer immunotherapy. CELL CHEMICAL BIOLOGY, 29(10), 1556-+. doi:10.1016/j.chembiol.2022.08.006

Xie, J., El Rami, F., Zhou, K., Simonetta, F., Chen, Z., Zheng, X., . . . Rao, J. (2022). Multiparameter Longitudinal Imaging of Immune Cell Activity in Chimeric Antigen Receptor T Cell and Checkpoint Blockade Therapies. ACS CENTRAL SCIENCE, 8(5), 590-602. doi:10.1021/acscentsci.2c00142


Xie, J., Mu, R., Fang, M., Cheng, Y., Senchyna, F., Moreno, A., . . . Rao, J. (2021). A dual-caged resorufin probe for rapid screening of infections resistant to lactam antibiotics. CHEMICAL SCIENCE, 12(26), 9153-9161. doi:10.1039/d1sc01471d

Chen, M., Chen, Z., Castillo, J. B., Cui, L., Zhou, K., Shen, B., . . . Rao, J. (2021). [18F]-C-SNAT4: an improved caspase-3-sensitive nanoaggregation PET tracer for imaging of tumor responses to chemo- and immunotherapies. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 48(11), 3386-3399. doi:10.1007/s00259-021-05297-0

Xie, J., Rice, M. A., Chen, Z., Cheng, Y., Hsu, E. -C., Chen, M., . . . Rao, J. (2021). In Vivo Imaging of Methionine Aminopeptidase II for Prostate Cancer Risk Stratification. CANCER RESEARCH, 81(9), 2510-2521. doi:10.1158/0008-5472.CAN-20-2969


Cui, L., Gouw, A. M., LaGory, E. L., Guo, S., Attarwala, N., Tang, Y., . . . Rao, J. (2021). Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. NATURE BIOTECHNOLOGY, 39(3), 357-367. doi:10.1038/s41587-020-0707-9

Dai, T., Xie, J., Zhu, Q., Kamariza, M., Jiang, K., Bertozzi, C. R., & Rao, J. (2020). A Fluorogenic Trehalose Probe for Tracking Phagocytosed Mycobacterium tuberculosis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 142(36), 15259-15264. doi:10.1021/jacs.0c07700

Chen, Z., Chen, M., Cheng, Y., Kowada, T., Xie, J., Zheng, X., & Rao, J. (2020). Exploring the Condensation Reaction between Aromatic Nitriles and Amino Thiols To Optimize In Situ Nanoparticle Formation for the Imaging of Proteases and Glycosidases in Cells. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 59(8), 3272-3279. doi:10.1002/anie.201913314


Cheng, Y., Xie, J., Lee, K. -H., Gaur, R. L., Song, A., Dai, T., . . . Rao, J. (2018). Rapid and specific labeling of single live Mycobacterium tuberculosis with a dual-targeting fluorogenic probe. SCIENCE TRANSLATIONAL MEDICINE, 10(454). doi:10.1126/scitranslmed.aar4470


Song, A., Cheng, Y., Xie, J., Banaei, N., & Rao, J. (2017). Intramolecular substitution uncages fluorogenic probes for detection of metallo-carbapenemase-expressing bacteria. CHEMICAL SCIENCE, 8(11), 7669-7674. doi:10.1039/c7sc02416a


Zheng, Y., Xie, J., Huang, X., Dong, J., Park, M. S., & Chan, W. K. (2016). Binding studies using Pichia pastoris expressed human aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins. PROTEIN EXPRESSION AND PURIFICATION, 122, 72-81. doi:10.1016/j.pep.2016.02.011


Penchala, S. C., Miller, M. R., Pal, A., Dong, J., Madadi, N. R., Xie, J., . . . Alhamadsheh, M. M. (2015). A biomimetic approach for enhancing the in vivo half-life of peptides. NATURE CHEMICAL BIOLOGY, 11(10), 793-+. doi:10.1038/NCHEMBIO.1907

Lyu, F., Xu, M., Cheng, Y., Xie, J., Rao, J., & Tang, S. K. Y. (2015). Quantitative detection of cells expressing BlaC using droplet-based microfluidics for use in the diagnosis of tuberculosis. BIOMICROFLUIDICS, 9(4). doi:10.1063/1.4928879


Xie, J., Huang, X., Park, M. S., Pham, H. M., & Chan, W. K. (2014). Differential suppression of the aryl hydrocarbon receptor nuclear translocator-dependent function by an aryl hydrocarbon receptor PAS-A-derived inhibitory molecule. BIOCHEMICAL PHARMACOLOGY, 88(2), 253-265. doi:10.1016/j.bcp.2014.01.021


Park, M. S., Chu, F., Xie, J., Wang, Y., Bhattacharya, P., & Chan, W. K. (2011). Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40. ANALYTICAL BIOCHEMISTRY, 410(2), 257-265. doi:10.1016/j.ab.2010.12.007